Anti-Human BNIP1 Polyclonal Antibody (HD183014)

Name: Anti-Human BNIP1 Polyclonal Antibody
Brand: abinScience
SKU: HD183014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD183014

Description

Anti-Human BNIP1 Polyclonal Antibody (HD183014) is a rabbit polyclonal antibody detecting BNIP1. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BNIP1 (Met1-Leu199).

Target

BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, BNIP1, NIP1, SEC20L, TRG-8, Transformation-related gene 8 protein, Vesicle transport protein SEC20

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q12981

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Vesicle transport protein SEC20 (BNIP1) is a ~26 kDa protein. As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).

1. Nakajima, K. et al. (2004) The EMBO journal 23, 3216-26. PMID: 15272311

2. Wang, P. et al. (2013) Cellular signalling 25, 2320-33. PMID: 23896122

3. Boyd, JM. et al. (1994) Cell 79, 341-51. PMID: 7954800

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-BNIP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-12 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: Mouse heart lysate
Lane 6: Mouse skeletal muscle lysate
Lane 7: Mouse testis lysate
Lane 8: Rat brain lysate
Lane 9: Rat heart lysate
Lane 10: Rat testis lysate

References

Datasheet

Anti-Human BNIP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BNIP1 Polyclonal Antibody (HD183014)

Overview

Images

References

Datasheet

SDS

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