Anti-Human CYP7A1 Polyclonal Antibody (HC204014)

Name: Anti-Human CYP7A1 Polyclonal Antibody
Brand: abinScience
SKU: HC204014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC204014

Description

Anti-Human CYP7A1 Polyclonal Antibody (HC204014) is a rabbit polyclonal antibody detecting CYP7A1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP7A1 (Gly43-Asp397).

Target

24-hydroxycholesterol 7-alpha-hydroxylase, CYP7, CYP7A1, CYPVII, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, Cytochrome P450 7A1, EC:1.14.14.23, EC:1.14.14.26

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P22680

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Cytochrome P450 7A1 (CYP7A1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol.

1. Norlin, M. et al. (2000) Journal of lipid research 41, 1629-39. PMID: 11013305

2. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124

3. Li, T. et al. (2010) Journal of lipid research 51, 832-42. PMID: 19965590

4. Shinkyo, R. et al. (2011) The Journal of biological chemistry 286, 33021-8. PMID: 21813643

5. Noshiro, M. et al. (1990) FEBS letters 268, 137-40. PMID: 2384150

Note

For research use only

Images

References

Datasheet

Anti-Human CYP7A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CYP7A1 Polyclonal Antibody (HC204014)

Overview

Images

References

Datasheet

SDS

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