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Anti-KASV RdRp Recombinant Antibody (SAA2809) (VK163013)

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Overview
Catalog No.VK163013
Description
Anti-KASV RdRp Recombinant Antibody (SAA2809) (VK163013) is a mouse monoclonal antibody detecting RdRp in ELISA. Suitable for Kasokero virus (KASV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityKasokero virus (KASV)
ApplicationsELISA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG
Clone IDSAA2809
Target EC:2.7.7.48, Large structural protein, RNA-directed RNA polymerase L, Replicase, Transcriptase
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession A0A0M5KLS1
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

RNA-directed RNA polymerase L is a ~253 kDa protein. RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped.

Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-KASV RdRp Recombinant Antibody (SAA2809)

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