Anti-Human ATR Polyclonal Antibody (HC586014)

Name: Anti-Human ATR Polyclonal Antibody
Brand: abinScience
SKU: HC586014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HC586014

Description

Anti-Human ATR Polyclonal Antibody (HC586014) is a rabbit polyclonal antibody detecting ATR in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATR (Ala2353-Met2644).

Target

ATR, Ataxia telangiectasia and Rad3-related protein, EC:2.7.11.1, FRAP-related protein 1, FRP1, Serine/threonine-protein kinase ATR

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13535

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase ATR is a ~301 kDa protein. Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination.

1. Hall-Jackson, CA. et al. (1999) Oncogene 18, 6707-13. PMID: 10597277

2. Kim, ST. et al. (1999) The Journal of biological chemistry 274, 37538-43. PMID: 10608806

3. Liu, Q. et al. (2000) Genes & development 14, 1448-59. PMID: 10859164

4. Cortez, D. et al. (2001) Science (New York, N.Y.) 294, 1713-6. PMID: 11721054

5. Zou, L. et al. (2003) Science (New York, N.Y.) 300, 1542-8. PMID: 12791985

6. Barr, SM. et al. (2003) Current biology : CB 13, 1047-51. PMID: 12814551

7. Wang, Y. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 15387-92. PMID: 14657349

8. Unsal-Kaçmaz, K. et al. (2004) Molecular and cellular biology 24, 1292-300. PMID: 14729973

9. Ward, IM. et al. (2004) The Journal of biological chemistry 279, 9677-80. PMID: 14742437

10. Cortez, D. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 10078-83. PMID: 15210935

Note

For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

References

Datasheet

Anti-Human ATR Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATR Polyclonal Antibody (HC586014)

Overview

Images

References

Datasheet

SDS