Anti-Human FoxP3 Polyclonal Antibody (HV388014)

Name: Anti-Human FoxP3 Polyclonal Antibody
Brand: abinScience
SKU: HV388014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV388014

Description

Anti-Human FoxP3 Polyclonal Antibody (HV388014) is a rabbit polyclonal antibody detecting FoxP3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human FOXP3 (Ser304-Pro431).

Target

FOXP3, Forkhead box protein P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, IPEX, Scurfin

Endotoxin level

Please contact with the lab for this information.

Observed Molecular Weight

55 kDa

Purification

Purified by antigen affinity column.

Accession

Q9BZS1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Forkhead box protein P3 (FOXP3) is a ~47 kDa protein. Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2.

1. Ono, M. et al. (2007) Nature 446, 685-9. PMID: 17377532

2. Bandukwala, HS. et al. (2011) Immunity 34, 479-91. PMID: 21458306

3. Vent-Schmidt, J. et al. (2014) International reviews of immunology 33, 110-28. PMID: 23947341

4. Passerini, L. et al. (2014) International reviews of immunology 33, 129-45. PMID: 24354325

5. Ramsdell, F. et al. (2014) Nature reviews. Immunology 14, 343-9. PMID: 24722479

6. Xiao, Y. et al. (2014) Cell reports 7, 1471-1480. PMID: 24835996

7. Becher, J. et al. (2018) Developmental cell 47, 592-607.e6. PMID: 30513302

8. Yang, J. et al. (2020) EMBO reports 21, e50308. PMID: 32644293

9. McMurchy, AN. et al. (2013) Blood 121, 1265-75. PMID: 23169781

10. Bettelli, E. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 5138-43. PMID: 15790681

Note

For research use only.

Images

References

Datasheet

Anti-Human FoxP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human FoxP3 Polyclonal Antibody (HV388014)

Overview

Images

References

Datasheet

SDS