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Anti-Mouse CCL2/MCP-1 Polyclonal Antibody (MY280014)

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Overview
Catalog No.MY280014
Description
Anti-Mouse CCL2/MCP-1 Polyclonal Antibody (MY280014) is a rabbit polyclonal antibody detecting CCL2/MCP-1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse CCL2/MCP-2 (Gln24-Arg96).
Target C-C motif chemokine 2, Ccl2, Je, MCP-1, Mcp1, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Scya2, Small-inducible cytokine A2
Endotoxin level Please contact with the lab for this information.
Observed Molecular Weight 20 kDa
Purification Purified by antigen affinity column.
Accession P10148
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

C-C motif chemokine 2 (HC11,MCP-1,MCP1,Monocyte chemotactic protein 1,Monocyte chemotactic and activating factor,Small-inducible cytokine A2,SCYA2,MCAF,Monocyte secretory protein JE,C-C motif chemokine 2,Monocyte chemoattractant protein 1,CCL2) is a ~11 kDa protein. Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

1. Hemmerich, S. et al. (1999) Biochemistry 38, 13013-25. PMID: 10529171
2. Jarnagin, K. et al. (1999) Biochemistry 38, 16167-77. PMID: 10587439
3. Paavola, CD. et al. (1998) The Journal of biological chemistry 273, 33157-65. PMID: 9837883
4. Zhang, YJ. et al. (1994) The Journal of biological chemistry 269, 15918-24. PMID: 8195247
5. Weber, M. et al. (1996) The Journal of experimental medicine 183, 681-5. PMID: 8627182
6. Chakravarty, L. et al. (1998) The Journal of biological chemistry 273, 29641-7. PMID: 9792674
7. Li, YS. et al. (1993) Molecular and cellular biochemistry 126, 61-8. PMID: 8107690
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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