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Anti-CcCoV-KY43 RBD Polyclonal Antibody (VK166014)

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Overview
Catalog No.VK166014
Description
Anti-CcCoV-KY43 RBD Polyclonal Antibody (VK166014) is a rabbit polyclonal antibody detecting RBD in ELISA, IHC, WB. Suitable for Cardioderma bat coronavirus (CcCoV-KY43).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityCardioderma bat coronavirus (CcCoV-KY43)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant CcCoV-KY43 RBD (Val496-Gly625).
Target spike protein [Cardioderma bat coronavirus], Coronavirus spike glycoprotein S1; pfam01600, SD-1 and SD-2 subdomains, the S1/S2 cleavage region, and the S2 fusion subunit of the spike (S) protein from alphacoronaviruses; cd22369, S1/S2 cleavage region, fusion peptide, internal fusion peptide, heptad repeat 1 [structural motif], heptad repeat 2 [structural motif]
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession WWB00503.1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Spike glycoprotein (RBD) is a ~139 kDa protein. May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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