Anti-Rat CCR2 Polyclonal Antibody (RW392014)

Name: Anti-Rat CCR2 Polyclonal Antibody
Brand: abinScience
SKU: RW392014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

RW392014

Description

Anti-Rat CCR2 Polyclonal Antibody (RW392014) is a rabbit polyclonal antibody detecting CCR2 in ELISA, IHC, WB. Suitable for Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Rat CD192/CCR2 (Met1-Pro60&Glu323-Leu373).

Target

C-C CKR-2, C-C chemokine receptor type 2, CC-CKR-2, CCR-2, CCR2, CD192, Ccr2, Cmkbr2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

O55193

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C-C chemokine receptor type 2 (CD192/CCR2) is a ~41 kDa protein. Key functional receptor for CCL2 but can also bind CCL7, and CCL12. Also transduces signaling mediated by CCL13. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion. Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation.

1. Tan, JHY. et al. (2013) The Journal of biological chemistry 288, 10024-10034. PMID: 23408426

2. Neehus, AL. et al. (2024) Cell 187, 390-408.e23. PMID: 38157855

3. Yamagami, S. et al. (1994) Biochemical and biophysical research communications 202, 1156-62. PMID: 8048929

4. Charo, IF. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 2752-6. PMID: 8146186

5. De Paula, VS. et al. (2013) Journal of molecular biology 425, 4479-95. PMID: 23938203

Note

For research use only.

Images

Western blot

Lane 1: U937 cell lysate
Lane 2: THP-1 cell lysate

References

Datasheet

Anti-Rat CCR2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Rat CCR2 Polyclonal Antibody (RW392014)

Overview

Images

References

Datasheet

SDS