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Anti-ZEBOV VP40 Polyclonal Antibody (VK783044)

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Anti-ZEBOV VP40 Polyclonal Antibody
Anti-ZEBOV VP40 Polyclonal Antibody
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Overview
Catalog No.VK783044
Description
Anti-ZEBOV VP40 Polyclonal Antibody (VK783044) is a rabbit polyclonal antibody detecting VP40 in ELISA, IHC, WB. Suitable for Zaire ebolavirus (ZEBOV) (Zaire Ebola virus).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityZaire ebolavirus (ZEBOV) (Zaire Ebola virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant ZEBOV VP40 (Asn31-Lys326).
Target Ebola VP40, Matrix protein VP40, Membrane-associated protein VP40, VP40, eVP40
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q77DJ6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein VP40 is a ~35 kDa protein. Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis).

Note For research use only.
Images
  • Anti-ZEBOV VP40 Polyclonal Antibody

    Western blot

    Lane 1: recombinant ZEBOV VP40 (Catalog No: VK783012)

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ZEBOV VP40 Polyclonal Antibody

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