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Anti-Mouse Integrin beta 3/CD61 Polyclonal Antibody (MY323014)

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Overview
Catalog No.MY323014
Description
Anti-Mouse Integrin beta 3/CD61 Polyclonal Antibody (MY323014) is a rabbit polyclonal antibody detecting Integrin beta 3/CD61 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse CD61/ITGB3 (Pro136-Ser378).
Target CD61, GP3A, GPIIIa, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O54890
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Integrin beta-3 (Integrin αVβ3 (CD51/ITGAV & CD61) is a ~87 kDa protein. Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen.

1. Si-Tahar, M. et al. (1997) The Journal of biological chemistry 272, 11636-47. PMID: 9111081
2. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
3. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099
4. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778
5. Mori, S. et al. (2008) The Journal of biological chemistry 283, 18066-75. PMID: 18441324
6. Mori, S. et al. (2017) Bioscience reports 37. PMID: 28302677
7. Saegusa, J. et al. (2009) The Journal of biological chemistry 284, 24106-14. PMID: 19578119
8. Cedano Prieto, DM. et al. (2017) PloS one 12, e0184285. PMID: 28873464
9. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430
10. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
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C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse Integrin beta 3/CD61 Polyclonal Antibody

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