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Anti-CSFV Glycoprotein E2 Polyclonal Antibody (VK750034)

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Overview
Catalog No.VK750034
Description
Anti-CSFV Glycoprotein E2 Polyclonal Antibody (VK750034) is a rabbit polyclonal antibody detecting Glycoprotein E2 in ELISA, IHC, WB. Suitable for Classical swine fever virus (CSFV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityClassical swine fever virus (CSFV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant CSFV E2 glycoprotein (Ser797-Lys989).
Target Autoprotease p20, Capsid protein C, Cysteine protease NS2, E(rns) glycoprotein, EC:2.7.7.48, EC:3.4.21.113, EC:3.4.22.-, EC:3.6.1.15, EC:3.6.4.13, Envelope glycoprotein E1, Envelope glycoprotein E2, Genome polyprotein, N-pro, N-terminal protease, NS2-3, NS4A, NS4B, NS5A, NS5B, Non-structural protein 2, Non-structural protein 2-3, Non-structural protein 3, Non-structural protein 4A, Non-structural protein 4B, Non-structural protein 5A, RNA-directed RNA polymerase, Serine protease NS3, Viroporin p7, gp33, gp44/48, gp55
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P21530
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Genome polyprotein (Glycoprotein E2) is a ~438 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-CSFV Glycoprotein E2 Polyclonal Antibody

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