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Anti-Mouse MPEG1 Polyclonal Antibody (MS702014)

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Overview
Catalog No.MS702014
Description
Anti-Mouse MPEG1 Polyclonal Antibody (MS702014) is a rabbit polyclonal antibody detecting MPEG1 in ELISA, IHC, WB. Suitable for Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse MPEG1 (Gly379-Lys576).
Target MPEG1, Macrophage gene 1 protein, Macrophage-expressed gene 1 protein, Macrophage-expressed gene 1 protein, processed form, Mpg-1, P-2, Perforin-2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession A1L314
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Macrophage-expressed gene 1 protein (MPEG1) is a ~78 kDa protein. Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide.

1. Fields, KA. et al. (2013) Infection and immunity 81, 3045-54. PMID: 23753625
2. McCormack, RM. et al. (2015) eLife 4. PMID: 26402460
3. McCormack, RM. et al. (2017) JCI insight 2. PMID: 28422754
4. Strbo, N. et al. (2019) Experimental dermatology 28, 225-232. PMID: 30609079
5. Pang, SS. et al. (2019) Nature communications 10, 4288. PMID: 31537793
6. Merselis, LC. et al. (2020) Frontiers in immunology 11, 601584. PMID: 33224153
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse MPEG1 Polyclonal Antibody

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