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Anti-N256-glycosylated CEACAM-5/CD66e Recombinant Antibody (EBC-129) (HY546083)

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Overview
Catalog No.HY546083
Description
Anti-N256-glycosylated CEACAM-5/CD66e Recombinant Antibody (EBC-129) (HY546083) is a human monoclonal antibody detecting CEACAM-5/CD66e in ELISA, Bioactivity: FCM, Functional assay, Research in vivo. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, Bioactivity: FCM, Functional assay, Research in vivo
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDEBC-129
Target CD66e, CEA, CEA cell adhesion molecule 5, CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Cell adhesion molecule CEACAM5, Meconium antigen 100
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P06731
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.

1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933
2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050
3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-N256-glycosylated CEACAM-5/CD66e Recombinant Antibody (EBC-129)

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