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Anti-Der f 1 Recombinant Antibody (SAA0174) (JN021033)

Anti-Der f 1 Recombinant Antibody (SAA0174)
Anti-Der f 1 Recombinant Antibody (SAA0174)
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Overview
Catalog No.JN021033
Species reactivityDermatophagoides farinae (American house dust mite)
ApplicationsELISA, WB
Host speciesHuman
IsotypeIgG1, kappa
Clone IDSAA0174
Clonality Monoclonal
Target Allergen Der f I, DERF1, Der f 1, EC:3.4.22.65, Major mite fecal allergen Der f 1, Peptidase 1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P16311
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only
Images
  • Anti-Der f 1 Recombinant Antibody (SAA0174)

    Western blot

    Western blot analysis was performed using anti-Der f 1 monoclonal antibody at 1 ug/mL on various samples.
    Lane 1: Recombinant Der f 1 protein (JN021011)
    Lane 2: Recombinant Der f 1 protein (JN021012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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