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Anti-RelA/NFkB p65 Recombinant Antibody (SRR1201) (HC453013)

Anti-RelA/NFkB p65 Recombinant Antibody (SRR1201)
Anti-RelA/NFkB p65 Recombinant Antibody (SRR1201)
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  • 100ul
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Overview
Catalog No.HC453013
Description
Anti-RelA/NFkB p65 Recombinant Antibody (SRR1201) (HC453013) is a rabbit monoclonal antibody detecting RelA/NFkB p65 in WB, IHC, IF, IP, FCM. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, FCM
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR1201
Tested applications WB, IHC, IF, IP, FCM
Target NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q04206, Q04207, Q7TQN4
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 50% glycerol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

RelA/NFkB p65 is a protein.

Note For research use only.
Isotype Control TF690107
Images
  • Anti-RelA/NFkB p65 Recombinant Antibody (SRR1201)

    Western blot

    Western blot analysis was performed using anti-NF-kB p65 antibody at 1:1000 on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: Raji cell lysate
    Lane 4: THP-1 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: Hela cell lysate
    Lane 7: MOLT-4 cell lysate
    Lane 8: NIH3T3 cell lysate
    Lane 9: HepG2 cell lysate
    Lane 10: MCF-7 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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