

| Catalog No. | HV181013 |
|---|---|
| Description |
Anti-TSG101 Recombinant Antibody (SRR1036) (HV181013) is a rabbit monoclonal antibody detecting TSG101 in WB, IHC, IF, IP, FCM. Suitable for Human, Mouse, Rat.
Highlights
|
| Species reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, FCM |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone ID | SRR1036 |
| Tested applications | WB, IHC, IF, IP, FCM |
| Target | ESCRT-I complex subunit TSG101, TSG101, Tumor susceptibility gene 101 protein |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | Q99816, Q61187, Q6IRE4 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4, 50% glycerol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. 1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413 2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426 |
| Note | For research use only. |
| Isotype Control | TF690107 |

Western blot analysis was performed using anti-TSG101 antibody at 1:1000 on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K562 cell lysate
Lane 5: NIH3T3 cell lysate
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