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Anti-TSG101 Recombinant Antibody (SRR1036) (HV181013)

Anti-TSG101 Recombinant Antibody (SRR1036)
Anti-TSG101 Recombinant Antibody (SRR1036)
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Overview
Catalog No.HV181013
Description
Anti-TSG101 Recombinant Antibody (SRR1036) (HV181013) is a rabbit monoclonal antibody detecting TSG101 in WB, IHC, IF, IP, FCM. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, FCM
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR1036
Tested applications WB, IHC, IF, IP, FCM
Target ESCRT-I complex subunit TSG101, TSG101, Tumor susceptibility gene 101 protein
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q99816, Q61187, Q6IRE4
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 50% glycerol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation.

1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426
Note For research use only.
Isotype Control TF690107
Images
  • Anti-TSG101 Recombinant Antibody (SRR1036)

    Western blot

    Western blot analysis was performed using anti-TSG101 antibody at 1:1000 on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K562 cell lysate
    Lane 5: NIH3T3 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Working Solution
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