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Anti-LC3B Recombinant Antibody (SRR0367) (HV519013)

Anti-LC3B Recombinant Antibody (SRR0367)
Anti-LC3B Recombinant Antibody (SRR0367)
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  • 100ul
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Overview
Catalog No.HV519013
Description
Anti-LC3B Recombinant Antibody (SRR0367) (HV519013) is a rabbit monoclonal antibody detecting LC3B in WB, IHC, IF, IP. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR0367
Tested applications WB, IHC, IF, IP
Target Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q9GZQ8, Q9CQV6, Q62625
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 50% glycerol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

LC3B is a protein.

Note For research use only.
Isotype Control TF690107
Images
  • Anti-LC3B Recombinant Antibody (SRR0367)

    Western blot

    Western blot analysis was performed using anti-LC3B antibody at 1:1000 on various samples.
    Lane 1: Hela cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: K562 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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