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Anti-Phospho-AKT (pSer473) Recombinant Antibody (SRR0405) (HB610043)

Anti-Phospho-AKT (pSer473) Recombinant Antibody (SRR0405)
Anti-Phospho-AKT (pSer473) Recombinant Antibody (SRR0405)
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Overview
Catalog No.HB610043
Description
Anti-Phospho-AKT (pSer473) Recombinant Antibody (SRR0405) (HB610043) is a rabbit monoclonal antibody detecting Phospho-AKT (pSer473) in WB, IHC, IF. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR0405
Tested applications WB, IHC, IF
Target AKT1, EC:2.7.11.1, PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, AKT2, EC:2.7.11.1, PKB beta, Protein kinase Akt-2, Protein kinase B beta, RAC protein kinase beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase, AKT3, EC:2.7.11.1, PKB gamma, PKBG, Protein kinase Akt-3, Protein kinase B gamma, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase, STK-2
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P31749, P31750, P47196, P31751, Q60823, P47197, Q9Y243, Q9WUA6, Q63484
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 50% glycerol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phospho-AKT (pSer473) is a protein.

Note For research use only.
Isotype Control TF690107
Images
  • Anti-Phospho-AKT (pSer473) Recombinant Antibody (SRR0405)

    Western blot

    Western blot analysis was performed using anti-p-AKT (S473) antibody at 1:1000 on various samples.
    Lane 1: 293T cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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