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Anti-Bcl-2 Recombinant Antibody (SRR0055) (HY131033)

Anti-Bcl-2 Recombinant Antibody (SRR0055)
Anti-Bcl-2 Recombinant Antibody (SRR0055)
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  • 100ul
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Overview
Catalog No.HY131033
Description
Anti-Bcl-2 Recombinant Antibody (SRR0055) (HY131033) is a rabbit monoclonal antibody detecting Bcl-2 in WB, IHC, IF, FCM. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsWB, IHC, IF, FCM
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR0055
Tested applications WB, IHC, IF, FCM
Target Apoptosis regulator Bcl-2, BCL2
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P10415, P10417, P49950
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 50% glycerol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bcl-2 is a protein.

Note For research use only.
Isotype Control TF690107
Images
  • Anti-Bcl-2 Recombinant Antibody (SRR0055)

    Western blot

    Western blot analysis was performed using anti-Bcl 2 antibody at 1:1000 on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: Raji cell lysate
    Lane 5: Mouse spleen lysate
    Lane 6: Mouse lung lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Mouse brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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