Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

Name: Anti-CD3 epsilon Recombinant Antibody (SRR2880)
Brand: abinScience
SKU: HY057073
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HY057073
Description	Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073) is a rabbit monoclonal antibody detecting CD3 epsilon in IF, FCM, WB. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	IF, FCM, WB
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR2880
Tested applications	IF, FCM, WB
Target	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P07766
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	T-cell surface glycoprotein CD3 epsilon chain (T3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,T-cell surface glycoprotein CD3 epsilon chain) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. 1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002 3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098 4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
Note	For research use only.

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Western blot

Western blot analysis was performed using anti-CD3 epsilon monoclonal antibody at 1 ug/mL on various samples.
Lane 1: Jurkat cell lysate
Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD3 epsilon (HY057073) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD3 epsilon was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD3 epsilon antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD3 epsilon monoclonal antibody (Catalog HY057073, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-CD3 epsilon Recombinant Antibody (SRR2880).pdf

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Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

Overview

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References

Datasheet

SDS