Anti-Human CD38 Recombinant Antibody (SRR0810) (HB691083)

Name: Anti-Human CD38 Recombinant Antibody (SRR0810)
Brand: abinScience
SKU: HB691083
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB691083
Description	Anti-Human CD38 Recombinant Antibody (SRR0810) (HB691083) is a rabbit monoclonal antibody detecting CD38 in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	IF, FCM
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR0810
Tested applications	IF, FCM
Target	2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, CD38, Cyclic ADP-ribose hydrolase 1, EC:2.4.99.20, EC:3.2.2.-, EC:3.2.2.6, T10, cADPR hydrolase 1
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P28907
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38/ADPRC 1) is a ~34 kDa protein. Multifunctional transmembrane glycoprotein able to exert enzymatic activities and also to mobilize calcium, to transduce signals, to adhere to hyaluronan and to other ligands. Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion. Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+). Has cADPR hydrolase activity. CD38 is the therapeutic target of daratumumab (Darzalex). 1. Tohgo, A. et al. (1994) The Journal of biological chemistry 269, 28555-7. PMID: 7961800 2. Takasawa, S. et al. (1993) The Journal of biological chemistry 268, 26052-4. PMID: 8253715 3. Moreschi, I. et al. (2006) Biochemical and biophysical research communications 345, 573-80. PMID: 16690024 4. Deaglio, S. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 395-402. PMID: 9551996 5. Frasca, L. et al. (2006) Blood 107, 2392-9. PMID: 16293598 6. Deaglio, S. et al. (2001) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 15, 580-2. PMID: 11259373
Note	For research use only.

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Immunofluorescence

Raji cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD38 (HB691083) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD38 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD38 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD38 monoclonal antibody (Catalog HB691083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD38 Recombinant Antibody (SRR0810).pdf

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Anti-Human CD38 Recombinant Antibody (SRR0810) (HB691083)

Overview

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References

Datasheet

SDS