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Anti-Human VEGF Recombinant Antibody (SRR0032) (HB941063)

Anti-Human VEGF Recombinant Antibody (SRR0032)
Anti-Human VEGF Recombinant Antibody (SRR0032)
Anti-Human VEGF Recombinant Antibody (SRR0032)
Anti-Human VEGF Recombinant Antibody (SRR0032)
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Overview
Catalog No.HB941063
Description
Anti-Human VEGF Recombinant Antibody (SRR0032) (HB941063) is a rabbit monoclonal antibody detecting VEGF in FCM. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityHuman
ApplicationsFCM
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSRR0032
Tested applications FCM
Target L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P15692
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vascular endothelial growth factor A, long form (Vascular endothelial growth factor A,VPF,VEGFA,VEGF,Vascular permeability factor,VEGF-A) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death.

1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969
Note For research use only.
Images
  • Anti-Human VEGF Recombinant Antibody (SRR0032)

    Flow cytometry

    Flow-cytometry using anti-Human VEGFA antibody. Permeabilized HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human VEGFA monoclonal antibody (Catalog HB941063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human VEGF Recombinant Antibody (SRR0032)

    Flow cytometry

    Flow-cytometry using anti-Human VEGFA antibody. Permeabilized U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human VEGFA monoclonal antibody (Catalog HB941063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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