Anti-Human CD46 Recombinant Antibody (SRR0011) (HB735023)

Name: Anti-Human CD46 Recombinant Antibody (SRR0011)
Brand: abinScience
SKU: HB735023
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB735023
Description	Anti-Human CD46 Recombinant Antibody (SRR0011) (HB735023) is a rabbit monoclonal antibody detecting CD46 in FCM, WB. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	FCM, WB
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR0011
Tested applications	FCM, WB
Target	CD46, MCP, MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15529
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Membrane cofactor protein (CD46) is a ~43 kDa protein. Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Note	For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD46monoclonal antibody at 1 ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK293T cell lysate
Flow cytometry

Flow-cytometry using anti-Human CD46 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD46 monoclonal antibody (Catalog HB735023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD46 Recombinant Antibody (SRR0011).pdf

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Formula

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Recombinant Proteins

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Anti-Human CD46 Recombinant Antibody (SRR0011) (HB735023)

Overview

Images

References

Datasheet

SDS