Anti-Human CD3 epsilon Recombinant Nanobody (SAA1330) (HY057083)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA1330)
Brand: abinScience
SKU: HY057083
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY057083

Description

Anti-Human CD3 epsilon Recombinant Nanobody (SAA1330) (HY057083) is a alpaca monoclonal antibody detecting CD3 epsilon in ELISA. Suitable for Homo sapiens (Human).

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

Species reactivity

Homo sapiens (Human)

Applications

ELISA

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-His

Clone ID

SAA1330

Target

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column

Accession

P07766

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell surface glycoprotein CD3 epsilon chain (T3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,T-cell surface glycoprotein CD3 epsilon chain) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.

1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

Note

For research use only

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References

Datasheet