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Anti-SMV Capsid Polyclonal Antibody (VK177014)

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Overview
Catalog No.VK177014
Description
Anti-SMV Capsid Polyclonal Antibody (VK177014) is a rabbit polyclonal antibody detecting Capsid in ELISA, IHC, WB. Suitable for Soybean mosaic virus (SMV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivitySoybean mosaic virus (SMV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant SMV Genome polyprotein (Ser2802-Gln3066).
Target CP, Capsid protein, Coat protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P21231
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Genome polyprotein (Capsid protein C/Core protein) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

1. Kuhn, RJ. et al. (2002) Cell 108, 717-25. PMID: 11893341
2. Sangiambut, S. et al. (2008) The Journal of general virology 89, 1254-1264. PMID: 18420804
3. Colpitts, TM. et al. (2011) PloS one 6, e24365. PMID: 21909430
4. Bhuvanakantham, R. et al. (2013) Cellular microbiology 15, 1688-706. PMID: 23522008
Note For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SMV Capsid Polyclonal Antibody

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