Anti-CD284/TLR4 Polyclonal Antibody (HT205014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HT205014

Description

Anti-CD284/TLR4 Polyclonal Antibody (HT205014) is a rabbit polyclonal antibody detecting CD284 in ELISA, IHC, WB. Suitable for Human, Pan paniscus, Papio anubis, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Gorilla gorilla gorilla, Pan paniscus, Papio anubis, Pongo pygmaeus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD284/TLR4 (Glu24-Lys631).

Target

Toll-like receptor 4, TLR4, CD284, hToll

Purification

Purified by antigen affinity column.

Accession

O00206

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toll-like receptor 4 (CD284/TLR4) is a ~95 kDa protein. Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production.

1. Arbour, NC. et al. (2000) Nature genetics 25, 187-91. PMID: 10835634

2. Bulut, Y. et al. (2005) The Journal of biological chemistry 280, 20961-7. PMID: 15809303

3. Jung, SB. et al. (2006) Infection and immunity 74, 2686-96. PMID: 16622205

4. Georgel, P. et al. (2007) Virology 362, 304-13. PMID: 17292937

5. Kim, F. et al. (2007) Circulation research 100, 1589-96. PMID: 17478729

6. Stewart, CR. et al. (2010) Nature immunology 11, 155-61. PMID: 20037584

7. Schmidt, M. et al. (2010) Nature immunology 11, 814-9. PMID: 20711192

8. Estruch, M. et al. (2013) Atherosclerosis 229, 356-62. PMID: 23880187

9. Tatematsu, M. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 3865-76. PMID: 27022195

10. Xiahou, Z. et al. (2017) Nature communications 8, 950. PMID: 29038465

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD284/TLR4 polyclonal antibody at 1ug/mL on recombinant CD284/TLR4 (Catalog No. HT205012).

References

Datasheet