Anti-SOCS2 Polyclonal Antibody (HT326014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HT326014

Description

Anti-SOCS2 Polyclonal Antibody (HT326014) is a rabbit polyclonal antibody detecting SOCS2 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Human, Mouse, Rat, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SOCS2 (Met1-Val198).

Target

CIS-2, STAT-induced STAT inhibitor 2, SSI-2, SOCS2, STATI2, CIS2, Cytokine-inducible SH2 protein 2, SSI2, Suppressor of cytokine signaling 2, SOCS-2

Purification

Purified by antigen affinity column.

Accession

O14508

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Suppressor of cytokine signaling 2 (SOCS2) is a ~22 kDa protein. Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as EPOR and GHR. Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination. The ECS(SOCS2) complex acts as a key regulator of growth hormone receptor (GHR) levels by mediating ubiquitination and degradation of GHR, following GHR phosphorylation by JAK2. The ECS(SOCS2) also catalyzes ubiquitination and degradation of JAK2-phosphorylated EPOR.

1. Eyckerman, S. et al. (2001) Nature cell biology 3, 1114-9. PMID: 11781573

2. Vesterlund, M. et al. (2011) PloS one 6, e25358. PMID: 21980433

3. Bulatov, E. et al. (2015) The Journal of biological chemistry 290, 4178-91. PMID: 25505247

4. Kung, WW. et al. (2019) Nature communications 10, 2534. PMID: 31182716

5. Linossi, EM. et al. (2021) Nature communications 12, 7032. PMID: 34857742

6. Ramachandran, S. et al. (2023) Nature communications 14, 6345. PMID: 37816714

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SOCS2 polyclonal antibody at 1ug/mL on recombinant SOCS2 (Catalog No. HT326012).

References

Datasheet

Anti-SOCS2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SOCS2 Polyclonal Antibody (HT326014)

Overview

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References

Datasheet

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