Anti-TBX3 Polyclonal Antibody (HT202014)

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Overview
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Datasheet

Overview

Catalog No.

HT202014

Description

Anti-TBX3 Polyclonal Antibody (HT202014) is a rabbit polyclonal antibody detecting TBX3 in ELISA, IHC, WB. Suitable for Mouse, Dog, Danio rerio, Chicken, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Dog, Danio rerio, Chicken, Human, Rat, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TBX3 (Leu107-Thr220).

Target

TBX3, T-box transcription factor TBX3, T-box protein 3

Purification

Purified by antigen affinity column.

Accession

O15119

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-box transcription factor TBX3 is a ~79 kDa protein. Transcriptional repressor involved in developmental processes. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes. Probably plays a role in limb pattern formation. Required for mammary placode induction, and maintenance of the mammary buds during development. Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2.

1. He, Ml. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 10212-7. PMID: 10468588

2. Lingbeek, ME. et al. (2002) The Journal of biological chemistry 277, 26120-7. PMID: 12000749

3. Martin, N. et al. (2012) The EMBO journal 31, 95-109. PMID: 22002537

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IBX3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse lung lysate
Lane 2: Rat lung lysate

References

Datasheet

Anti-TBX3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TBX3 Polyclonal Antibody (HT202014)

Overview

Images

References

Datasheet

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