Anti-PLSCR1 Polyclonal Antibody (HT453014)

Name: Anti-PLSCR1 Polyclonal Antibody
Brand: abinScience
SKU: HT453014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HT453014

Description

Anti-PLSCR1 Polyclonal Antibody (HT453014) is a rabbit polyclonal antibody detecting PLSCR1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PLSCR1 (Met1-Trp318).

Target

Mg(2+)-dependent nuclease, Erythrocyte phospholipid scramblase, PLSCR1, PL scramblase 1, Phospholipid scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipid scramblase 1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O15162

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phospholipid scramblase 1 (PLSCR1) is a ~35 kDa protein. Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface. Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5. Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A. Negatively regulates FcR-mediated phagocytosis in differentiated macrophages. May contribute to cytokine-regulated cell proliferation and differentiation.

1. Frasch, SC. et al. (2000) The Journal of biological chemistry 275, 23065-73. PMID: 10770950

2. Sahu, SK. et al. (2008) Biotechnology letters 30, 2131-7. PMID: 18629440

3. Francis, VG. et al. (2013) The FEBS journal 280, 2855-69. PMID: 23590222

4. Shettihalli, AK. et al. (2013) Chemical research in toxicology 26, 918-25. PMID: 23659204

5. Sánchez-Magraner, L. et al. (2014) The Journal of membrane biology 247, 155-65. PMID: 24343571

6. Rayala, S. et al. (2014) The Journal of biological chemistry 289, 13206-18. PMID: 24648509

7. Ahyayauch, H. et al. (2018) Scientific reports 8, 7456. PMID: 29748552

8. Guo, J. et al. (2020) Cells 9. PMID: 32110987

9. Bassé, F. et al. (1996) The Journal of biological chemistry 271, 17205-10. PMID: 8663431

10. Zhou, Q. et al. (1997) The Journal of biological chemistry 272, 18240-4. PMID: 9218461

Note

For research use only.

Images

References

Datasheet

Anti-PLSCR1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-PLSCR1 Polyclonal Antibody (HT453014)

Overview

Images

References

Datasheet

SDS

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