Anti-ALDH1A1 Polyclonal Antibody (HF669014)

Name: Anti-ALDH1A1 Polyclonal Antibody
Brand: abinScience
SKU: HF669014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF669014

Description

Anti-ALDH1A1 Polyclonal Antibody (HF669014) is a rabbit polyclonal antibody detecting ALDH1A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALDH1A1 (Met1-Ser501).

Target

Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1, ALDH-E1, RalDH1, Aldehyde dehydrogenase family 1 member A1, ALDH1A1, ALDH1, RALDH 1, ALDC, ALHDII, PUMB1

Purification

Purified by antigen affinity column.

Accession

P00352

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Aldehyde dehydrogenase 1A1 (ALDH1A1) is a ~54 kDa protein. Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification.

1. Manzer, R. et al. (2003) DNA and cell biology 22, 329-38. PMID: 12941160

2. Choudhary, S. et al. (2005) Investigative ophthalmology & visual science 46, 259-67. PMID: 15623782

3. Collard, F. et al. (2007) Biochimie 89, 369-73. PMID: 17175089

4. Xiao, T. et al. (2009) Journal of toxicology and environmental health. Part A 72, 577-84. PMID: 19296407

5. Morgan, CA. et al. (2015) Chemico-biological interactions 234, 29-37. PMID: 25450233

6. Koch, MF. et al. (2015) Angewandte Chemie (International ed. in English) 54, 13550-4. PMID: 26373694

Note

For research use only.

Images

References

Datasheet

Anti-ALDH1A1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ALDH1A1 Polyclonal Antibody (HF669014)

Overview

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References

Datasheet

SDS

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