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Anti-OTC Polyclonal Antibody (HF835014)

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Anti-OTC Polyclonal Antibody
Anti-OTC Polyclonal Antibody
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Overview
Catalog No.HF835014
Description
Anti-OTC Polyclonal Antibody (HF835014) is a rabbit polyclonal antibody detecting OTC in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Ovis aries, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human OTC (Gln78-Leu342).
Target OTC, Ornithine carbamoyltransferase, mitochondrial, OTCase, Ornithine transcarbamylase, mitochondrial
Purification Purified by antigen affinity column.
Accession P00480
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ornithine transcarbamylase, mitochondrial (OTC) is a ~39 kDa protein. Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.

1. Lee, JT. et al. (1989) The Journal of clinical investigation 84, 1762-6. PMID: 2556444
2. Horwich, AL. et al. (1984) Science (New York, N.Y.) 224, 1068-74. PMID: 6372096
3. Matsuura, T. et al. (1994) Human genetics 93, 129-34. PMID: 8112735
Note For research use only.
Images
  • Anti-OTC Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-OTC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse colon lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-OTC Polyclonal Antibody

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