Anti-F2 Polyclonal Antibody (HF621014)

Name: Anti-F2 Polyclonal Antibody
Brand: abinScience
SKU: HF621014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF621014

Description

Anti-F2 Polyclonal Antibody (HF621014) is a rabbit polyclonal antibody detecting F2 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human F2 (Ala44-Arg327).

Target

F2, Coagulation factor II, Prothrombin

Purification

Purified by antigen affinity column.

Accession

P00734

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Prothrombin (F2/PIVKA-II) is a ~70 kDa protein. Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells.

1. Naito, K. et al. (1991) The Journal of biological chemistry 266, 7353-8. PMID: 2019570

2. Choi, SH. et al. (2011) Blood 118, 6963-70. PMID: 21976677

3. Shinozawa, E. et al. (2018) Frontiers in pharmacology 9, 1431. PMID: 30568593

4. Senden, NH. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 4318-24. PMID: 9780208

Note

For research use only.

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