Anti-EGF Polyclonal Antibody (HF557014)

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Datasheet

Overview

Catalog No.

HF557014

Description

Anti-EGF Polyclonal Antibody (HF557014) is a rabbit polyclonal antibody detecting EGF in ELISA, IHC, WB. Suitable for Human, Cat, Dog, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Cat, Dog, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EGF (Met608-Gly751).

Target

Urogastrone, Pro-epidermal growth factor, EGF

Purification

Purified by antigen affinity column.

Accession

P01133

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pro-epidermal growth factor (EGF) is a ~133 kDa protein. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.

1. Hermann, PM. et al. (2000) The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 6355-64. PMID: 10964941

Note

For research use only.

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