Anti-HLA-DRA Polyclonal Antibody (HF884014)

Name: Anti-HLA-DRA Polyclonal Antibody
Brand: abinScience
SKU: HF884014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF884014

Description

Anti-HLA-DRA Polyclonal Antibody (HF884014) is a rabbit polyclonal antibody detecting HLA-DRA in ELISA, IHC, WB. Suitable for Human and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human HLA-DRA (Lys27-Glu216).

Target

HLA-DRA1, MHC class II antigen DRA, HLA-DRA, HLA class II histocompatibility antigen, DR alpha chain

Purification

Purified by antigen affinity column.

Accession

P01903

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) is a ~28 kDa protein. An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins.

1. Shams, H. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 1966-77. PMID: 15265931

2. Röhn, TA. et al. (2004) Nature immunology 5, 909-18. PMID: 15322540

3. Deng, L. et al. (2007) Nature immunology 8, 398-408. PMID: 17334368

4. Kwok, WW. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 188, 2537-44. PMID: 22327072

5. Scally, SW. et al. (2013) The Journal of experimental medicine 210, 2569-82. PMID: 24190431

6. Muehling, LM. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 197, 3214-3224. PMID: 27591323

7. Galperin, M. et al. (2018) Science immunology 3. PMID: 29884618

8. Abelin, JG. et al. (2019) Immunity 51, 766-779.e17. PMID: 31495665

9. Stern, LJ. et al. (1994) Nature 368, 215-21. PMID: 8145819

10. Kropshofer, H. et al. (1997) Immunity 6, 293-302. PMID: 9075930

Note

For research use only.

Images

References

Datasheet

Anti-HLA-DRA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-HLA-DRA Polyclonal Antibody (HF884014)

Overview

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References

Datasheet

SDS

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