Anti-ANG Polyclonal Antibody (HY052014)

Name: Anti-ANG Polyclonal Antibody
Brand: abinScience
SKU: HY052014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY052014

Description

Anti-ANG Polyclonal Antibody (HY052014) is a rabbit polyclonal antibody detecting ANG in ELISA, IHC, WB. Suitable for Human, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ANG (Gln25-Pro147).

Target

Ribonuclease 5, Angiogenin, RNase 5, RNASE5, ANG

Purification

Purified by antigen affinity column.

Accession

P03950

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36.

1. Xu, ZP. et al. (2002) Biochemical and biophysical research communications 294, 287-92. PMID: 12051708

2. Saxena, SK. et al. (1992) The Journal of biological chemistry 267, 21982-6. PMID: 1400510

3. Yamasaki, S. et al. (2009) The Journal of cell biology 185, 35-42. PMID: 19332886

4. Emara, MM. et al. (2010) The Journal of biological chemistry 285, 10959-68. PMID: 20129916

5. Ivanov, P. et al. (2011) Molecular cell 43, 613-23. PMID: 21855800

6. Thiyagarajan, N. et al. (2012) Nature communications 3, 1121. PMID: 23047679

7. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625

8. Shapiro, R. et al. (1986) Biochemistry 25, 3527-32. PMID: 2424496

9. Harper, JW. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 7139-43. PMID: 2459697

10. Lee, FS. et al. (1989) Biochemical and biophysical research communications 161, 121-6. PMID: 2730651

Note

For research use only.

Images

References

Datasheet

Anti-ANG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-ANG Polyclonal Antibody (HY052014)

Overview

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References

Datasheet

SDS

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