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Anti-PDGFA Polyclonal Antibody (HY061014)

Anti-PDGFA Polyclonal Antibody
Anti-PDGFA Polyclonal Antibody
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Overview
Catalog No.HY061014
Description
Anti-PDGFA Polyclonal Antibody (HY061014) is a rabbit polyclonal antibody detecting PDGFA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PDGFA (Ser87-Thr211).
Target Platelet-derived growth factor alpha polypeptide, Platelet-derived growth factor subunit A, Platelet-derived growth factor A chain, PDGF subunit A, PDGFA, PDGF-1, PDGF1
Purification Purified by antigen affinity column.
Accession P04085
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Platelet-derived growth factor subunit A (PDGFA) is a ~24 kDa protein. Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing.

Note For research use only.
Images
  • Anti-PDGFA Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PDGFA polyclonal antibody at 1ug/mL on recombinant PDGFA (Catalog No. HY061012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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