Catalog No. | HY178014 | ||||||||
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Species reactivity | Human, Mouse, Rat, Bos taurus, Oryctolagus cuniculus, Pig | ||||||||
Applications | ELISA, IHC, WB | ||||||||
Host species | Rabbit | ||||||||
Isotype | IgG | ||||||||
Clonality | Polyclonal | ||||||||
Immunogen | E. coli - derived recombinant Human CAPNS1 (Met1-Ser268). | ||||||||
Target | CAPN4, CAPNS1, Calpain regulatory subunit, Calcium-dependent protease small subunit 1, Calcium-dependent protease small subunit, Calcium-activated neutral proteinase small subunit, CDPS, CSS1, Calpain small subunit 1, CANP small subunit, CAPNS | ||||||||
Purification | Purified by antigen affinity column. | ||||||||
Accession | P04632 | ||||||||
Form | Liquid | ||||||||
Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
Product Usage Information |
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Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
Note | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CAPNS1 (HY178014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CAPNS1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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