Catalog No. | HY178014 |
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Species reactivity | Human, Mouse, Rat, Bos taurus, Oryctolagus cuniculus, Pig |
Applications | ELISA, IHC, WB |
Host species | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | E. coli - derived recombinant Human CAPNS1 (Met1-Ser268). |
Target | CAPN4,CAPNS1,Calpain regulatory subunit,Calcium-dependent protease small subunit 1,Calcium-dependent protease small subunit,Calcium-activated neutral proteinase small subunit,CDPS,CSS1,Calpain small subunit 1,CANP small subunit,CAPNS |
Purification | Purified by antigen affinity column. |
Accession | P04632 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
Note | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CAPNS1 (HY178014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CAPNS1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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