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Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014)

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Overview
Catalog No.HY322014
Description
Anti-HSPB1/HSP27 Polyclonal Antibody (HY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Human and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HSPB1/HSP27 (Met1-Lys205).
Target Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, SRP27, Heat shock protein beta-1, Stress-responsive protein 27, HspB1, 28 kDa heat shock protein, HSPB1, HSP27, HSP28, HSP 27
Purification Purified by antigen affinity column.
Accession P04792
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Heat shock protein beta-1 (HSPB1/HSP27) is a ~22 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Rogalla, T. et al. (1999) The Journal of biological chemistry 274, 18947-56. PMID: 10383393
2. Almeida-Souza, L. et al. (2010) The Journal of biological chemistry 285, 12778-86. PMID: 20178975
3. Kostenko, S. et al. (2009) Cellular signalling 21, 712-8. PMID: 19166925
4. Holmgren, A. et al. (2013) Acta neuropathologica 126, 93-108. PMID: 23728742
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HSPB1/HSP27 Polyclonal Antibody

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