Anti-RB1 Polyclonal Antibody (HY189014)

Name: Anti-RB1 Polyclonal Antibody
Brand: abinScience
SKU: HY189014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY189014

Description

Anti-RB1 Polyclonal Antibody (HY189014) is a rabbit polyclonal antibody detecting RB1 in ELISA, IHC, WB. Suitable for Mouse, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RB1 (Gln639-Thr778).

Target

pRb, p110-RB1, Retinoblastoma-associated protein, Rb, p105-Rb, RB1, pp110

Purification

Purified by antigen affinity column.

Accession

P06400

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Retinoblastoma-associated protein (RB1) is a ~106 kDa protein. Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C.

1. Harbour, JW. et al. (1999) Cell 98, 859-69. PMID: 10499802

2. Ren, S. et al. (2004) Cell 117, 239-51. PMID: 15084261

Note

For research use only.

Images

References

Datasheet

Anti-RB1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RB1 Polyclonal Antibody (HY189014)

Overview

Images

References

Datasheet

SDS

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