Anti-RARA Polyclonal Antibody (HY483014)

Name: Anti-RARA Polyclonal Antibody
Brand: abinScience
SKU: HY483014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY483014

Description

Anti-RARA Polyclonal Antibody (HY483014) is a rabbit polyclonal antibody detecting RARA in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Coturnix japonica, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RARA (Ser68-Glu173).

Target

RARA, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1, RAR-alpha, NR1B1

Purification

Purified by antigen affinity column.

Accession

P10276

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Retinoic acid receptor alpha (RARA) is a ~50 kDa protein. Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation.

1. Srinivas, H. et al. (2006) The Biochemical journal 395, 653-62. PMID: 16417524

2. Zhu, L. et al. (2009) Endocrinology 150, 5586-95. PMID: 19850744

3. Santos, NC. et al. (2010) Endocrinology 151, 2361-72. PMID: 20215566

4. Sato, Y. et al. (2010) PloS one 5, e15119. PMID: 21152046

5. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846

6. Lee, DY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 2072-2077. PMID: 28167758

7. Qin, Z. et al. (2009) Molecular and cellular biology 29, 3633-43. PMID: 19398580

Note

For research use only.

Images

References

Datasheet

Anti-RARA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-RARA Polyclonal Antibody (HY483014)

Overview

Images

References

Datasheet

SDS

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