Anti-CD11b/ITGAM Polyclonal Antibody (HY474014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HY474014

Description

Anti-CD11b/ITGAM Polyclonal Antibody (HY474014) is a rabbit polyclonal antibody detecting ITGAM in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD11b/ITGAM (Gln133-Gly337).

Target

CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, ITGAM, CD11b, CD11 antigen-like family member B, Integrin alpha-M, CR3A, CD11B, Neutrophil adherence receptor

Purification

Purified by antigen affinity column.

Accession

P11215

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Integrin alpha-M (CD11b/ITGAM) is a ~127 kDa protein. Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

1. Losse, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 912-21. PMID: 20008295

2. DiScipio, RG. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 4057-66. PMID: 9558116

3. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980

4. Jerke, U. et al. (2011) The Journal of biological chemistry 286, 7070-81. PMID: 21193407

5. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD11b/ITGAM polyclonal antibody at 1ug/mL on recombinant CD11b/ITGAM (Catalog No. HY474012).

References

Datasheet

Anti-CD11b/ITGAM Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD11b/ITGAM Polyclonal Antibody (HY474014)

Overview

Images

References

Datasheet

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