Anti-CDK4 Polyclonal Antibody (HY215014)

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50ug
100ug

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Overview
Images
References
Datasheet

Overview

Catalog No.

HY215014

Description

Anti-CDK4 Polyclonal Antibody (HY215014) is a rabbit polyclonal antibody detecting CDK4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Ovis aries.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Ovis aries, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDK4 (Met1-Glu303).

Target

Cell division protein kinase 4, PSK-J3, Cyclin-dependent kinase 4, CDK4

Purification

Purified by antigen affinity column.

Accession

P11802

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

References

Circular RNA CLASP1 modulates the GLI1/SNAIL axis and enhances macrophage polarization in breast cancer.[HY215014]

Note

For research use only.

Images

Western blot

Western blot analysis of CCND1 and CDK4 protein levels in MDA-MB-231 and MCF-7 cells transfected with sh-circCLASP1 or sh-scramble.
Western blot

Western blot analysis was performed using anti-CDK4 polyclonal antibody at 1ug/mL on recombinant CDK4 (Catalog No. HY215012).