Anti-IL7 Polyclonal Antibody (HY408014)

Name: Anti-IL7 Polyclonal Antibody
Brand: abinScience
SKU: HY408014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY408014

Description

Anti-IL7 Polyclonal Antibody (HY408014) is a rabbit polyclonal antibody detecting IL7 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL7 (Asp26-His177).

Target

IL-7, Interleukin-7, IL7

Purification

Purified by antigen affinity column.

Accession

P13232

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-7 (IL7) is a ~20 kDa protein. Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

1. Bazdar, DA. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4698-704. PMID: 25870237

2. Dittel, BN. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 58-67. PMID: 7527823

3. Kondo, M. et al. (1994) Science (New York, N.Y.) 263, 1453-4. PMID: 8128231

4. Johnson, SE. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 8109-17. PMID: 18523275

5. Rochman, Y. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 19455-60. PMID: 20974963

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL7 polyclonal antibody at 1ug/mL on recombinant IL7 (Catalog No. HY408012).

References

Datasheet

Anti-IL7 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IL7 Polyclonal Antibody (HY408014)

Overview

Images

References

Datasheet

SDS

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