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Anti-ACP3 Polyclonal Antibody (HB187014)

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Overview
Catalog No.HB187014
Description
Anti-ACP3 Polyclonal Antibody (HB187014) is a rabbit polyclonal antibody detecting ACP3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ACP3 (Leu167-Thr335).
Target Prostatic acid phosphatase, Thiamine monophosphatase, ACPP, TMPase, ACP3, Protein tyrosine phosphatase ACP3, 5'-nucleotidase, Acid phosphatase 3, PAP, 5'-NT, Ecto-5'-nucleotidase
Purification Purified by antigen affinity column.
Accession P15309
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Prostatic acid phosphatase (ACP3) is a ~44 kDa protein. A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.

1. Hiroyama, M. et al. (1999) The Journal of biological chemistry 274, 29172-80. PMID: 10506173
2. Tanaka, M. et al. (2004) FEBS letters 571, 197-204. PMID: 15280042
3. Chuang, TD. et al. (2010) The Journal of biological chemistry 285, 23598-606. PMID: 20498373
4. Zhang, Z. et al. (1997) Acta biochimica Polonica 44, 659-72. PMID: 9584846
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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