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Anti-CEBPB Polyclonal Antibody (HB194014)

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Overview
Catalog No.HB194014
Description
Anti-CEBPB Polyclonal Antibody (HB194014) is a rabbit polyclonal antibody detecting CEBPB in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CEBPB (Met199-Cys345).
Target Liver activator protein, Transcription factor 5, LIP, LAP, Liver-enriched inhibitory protein, TCF5, Nuclear factor NF-IL6, C/EBP beta, TCF-5, CCAAT/enhancer-binding protein beta, CEBPB
Purification Purified by antigen affinity column.
Accession P17676
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

CCAAT/enhancer-binding protein beta (CEBPB) is a ~36 kDa protein. Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Also plays a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant roles with CEBPA.

1. Roy, SK. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 7945-50. PMID: 12048245
2. Kinoshita, S. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 1473-6. PMID: 1741402
3. Pless, O. et al. (2008) The Journal of biological chemistry 283, 26357-63. PMID: 18647749
4. Chinery, R. et al. (1997) The Journal of biological chemistry 272, 30356-61. PMID: 9374525
5. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CEBPB Polyclonal Antibody

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