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Anti-PTBP1 Polyclonal Antibody (HB835014)

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Overview
Catalog No.HB835014
Description
Anti-PTBP1 Polyclonal Antibody (HB835014) is a rabbit polyclonal antibody detecting PTBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Rat, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PTBP1 (Met1-Ile531).
Target PTB, Polypyrimidine tract-binding protein 1, 57 kDa RNA-binding protein PPTB-1, PTBP1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I
Purification Purified by antigen affinity column.
Accession P26599
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polypyrimidine tract-binding protein 1 (PTBP1) is a ~59 kDa protein. Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA.

1. Wang, J. et al. (2004) Journal of neurochemistry 88, 1078-90. PMID: 15009664
2. Zuccato, E. et al. (2004) The Journal of biological chemistry 279, 16980-8. PMID: 14966131
3. David, CJ. et al. (2010) Nature 463, 364-8. PMID: 20010808
4. Kafasla, P. et al. (2011) RNA (New York, N.Y.) 17, 1120-31. PMID: 21518806
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PTBP1 Polyclonal Antibody

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