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Anti-PRDX3 Polyclonal Antibody (HB236014)

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Overview
Catalog No.HB236014
Description
Anti-PRDX3 Polyclonal Antibody (HB236014) is a rabbit polyclonal antibody detecting PRDX3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PRDX3 (Val5-Trp233).
Target HBC189, AOP1, Antioxidant protein 1, Peroxiredoxin III, AOP-1, Protein MER5 homolog, Prx-III, Peroxiredoxin-3, Thioredoxin-dependent peroxide reductase, mitochondrial, Thioredoxin-dependent peroxiredoxin 3, PRDX3
Purification Purified by antigen affinity column.
Accession P30048
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) is a ~27 kDa protein. Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Required for the maintenance of physical strength.

1. Cao, Z. et al. (2007) Journal of molecular biology 372, 1022-1033. PMID: 17707404
2. Yewdall, NA. et al. (2018) Biochemical and biophysical research communications 497, 558-563. PMID: 29438714
3. Rebelo, AP. et al. (2021) Brain : a journal of neurology 144, 1467-1481. PMID: 33889951
4. Tsuji, K. et al. (1995) The Biochemical journal 307 ( Pt 2), 377-81. PMID: 7733872
5. Masaki, M. et al. (2003) European journal of biochemistry 270, 76-83. PMID: 12492477
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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