Anti-STIP1 Polyclonal Antibody (HB235014)

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Datasheet

Overview

Catalog No.

HB235014

Description

Anti-STIP1 Polyclonal Antibody (HB235014) is a rabbit polyclonal antibody detecting STIP1 in ELISA, IHC, WB. Suitable for Bovine, Cricetulus griseus, Human, Macaca fascicularis, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Cricetulus griseus, Human, Macaca fascicularis, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STIP1 (Met1-Lys169).

Target

Hop, Transformation-sensitive protein IEF SSP 3521, Hsc70/Hsp90-organizing protein, Renal carcinoma antigen NY-REN-11, Stress-induced-phosphoprotein 1, STI1, STIP1

Purification

Purified by antigen affinity column.

Accession

P31948

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Stress-induced-phosphoprotein 1 (STIP1) is a ~62 kDa protein. Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.

1. Woodford, MR. et al. (2016) Nature communications 7, 12037. PMID: 27353360

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-STIP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: BXPC3 cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Lncap cell lysate
Lane 6: Jurkat cell lysate
Lane 7: K562 cell lysate
Lane 8: NIH3T3 cell lysate

References

Datasheet

Anti-STIP1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-STIP1 Polyclonal Antibody (HB235014)

Overview

Images

References

Datasheet

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