Anti-MAP2K2 Polyclonal Antibody (HW411014)

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100ug

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Datasheet

Overview

Catalog No.

HW411014

Description

Anti-MAP2K2 Polyclonal Antibody (HW411014) is a rabbit polyclonal antibody detecting MAP2K2 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Dog, Rat, Cricetulus griseus, Cyprinus carpio, Chicken, Oryctolagus cuniculus, Serinus canaria, Xenopus laevis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAP2K2 (Val86-Pro331).

Target

MAP kinase kinase 2, MAPK/ERK kinase 2, ERK activator kinase 2, MAPKK 2, PRKMK2, MAP2K2, MEK 2, MEK2, MKK2, Dual specificity mitogen-activated protein kinase kinase 2

Purification

Purified by antigen affinity column.

Accession

P36507

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dual specificity mitogen-activated protein kinase kinase 2 (MAP2K2) is a ~44 kDa protein. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.

1. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAP2K2 polyclonal antibody at 1ug/mL on recombinant MAP2K2 (Catalog No. HW411012).

References

Datasheet

Anti-MAP2K2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MAP2K2 Polyclonal Antibody (HW411014)

Overview

Images

References

Datasheet

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