Anti-Human DSG3 Recombinant Antibody (DF151) (HW575107)

Name: Anti-Human DSG3 Recombinant Antibody (DF151)
Brand: abinScience
SKU: HW575107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HW575107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	DF151
Clonality	Monoclonal
Target	Cadherin family member 6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, CDHF6
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P32926
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3. 1. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537 2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552 3. Tsang, SM. et al. (2012) The Journal of pathology 227, 81-93. PMID: 22294297 4. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473 5. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450
Note	For research use only.

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Bioactivity

Detects DSG3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human DSG3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DSG3 antibody monoclonal antibody (Catalog # HW575107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-human DSG3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human DSG3 (Catalog No: HW575011)
Lane 2: negative control

References

Datasheet

Anti-Human DSG3 Recombinant Antibody (DF151).pdf

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Anti-Human DSG3 Recombinant Antibody (DF151) (HW575107)

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